Lekha A.

Bioscience Researcher

Bioscience researcher with more than 20 years of R & D experience in proteomics and cell based therapeutics. Hold a masters in Biotechnology and Ph.D. in Biochemistry. Key research areas include protein / antibody purification and characterisation, antibody labelling, design and development of biomarkers and cell-based assays for equine application. Strong foundation in bio-analytical method validation, especially on biomarkers relevant to inflammation, oxidative stress, regeneration and bone-metabolism. Established novel macrophage therapy for treatment of musculo-skeletal injuries in racehorses, actively involved in R & D of equine regenerative therapies which include Orthokine, Mesenchymal stem cell (MSC) therapy and platelet rich plasma (PRP) therapy. Developed a panel of cell based assays using High content imaging platform, to monitor the innate and adaptive immune function. These assays shed a new perspective on performance development in elite race horses and were extended to monitor fertility and reproductive performance. Established the pipeline for recombinant equine therapeutic protein production using mammalian cell culture. Developed and optimised upstream and downstream protein production using HEK-293 cells, that could be scaled up to a 20 L capacity. Established recombinant protein purification using AKTA systems and supported the equine clinical trials for testing the efficacy of the purified protein. Developed and patented novel skin care formulations based on in-depth research, lab experiments and cell based assays to assess the action mechanism of topical drugs on immune cells. Important products developed include HoofCream (patented, EP3305288 AI), moringa cream, henna cream, amnion cream, dermatitis cream, autologous serum based creams for dermal wound healing. Ability to combine research and creativity, provide virtual technical support on material and device sourcing, prototype development, scale up, QC development and stability testing of topical formulations. Proactively scans new research concepts, materials and technical opportunities. Ability to select and blend functional ingredients for specific product requirements. A self-motivated scientific writer and editor with a positive attitude, excellent organisational skills and loves varied assignments. Extensive experience in systematic literature analysis to support evidence-based decision making process. Ability to critically appraise evidence, interpret complex scientific data, detect shortcomings, recognise opportunities and develop knowledge and insights to support evidence based answers. Experience in systematic literature analysis and meta-analysis using R for clinical evidence, ensure that the work is scientifically accurate and fully referenced. Broad knowledge of medical and scientific topics with experience in comparing laboratory devices, diagnostic kits, analytical methods, assay validation and is confident to work on these areas Team player with strong work ethic and commitment to deliver quality work on time. Passionate about lifelong learning and self-improvement.