Najam U S.

I) Bioinformatics services: Using Galaxy or setting up pipeline on R studio: 1 RNA Seq Data Analysis: Aligning RNA-seq data (Tophat or Bowtie) DESeq Gene Set Enrichment Analysis with ClusterProfiler Generating plots like Volcano Plots and Heat maps for differentially expressed genes 2 ChipSeq analysis: Deeptools2 bamCoverage Deeptools2 computeMatrix and plotHeatmap using BioSAILs 3 Variant Calling Identifying single nucleotide polymorphisms (SNPs) and small insertions and deletion (indels) from NGS data 4 Metagenomics Quality Control Shotgun Metagenomics Taxonomic Classification Functional Analysis 5 Single cell RNA sequencing Loading the data Cell QC Data normalization and PCA Cell clustering 6 Computer-Aided Drug Design MD simulation Molecular Docking by Schrodinger Gromacs 7 Chromosome conformation techniques (3D genome organization): Micro C-XL, HiC data analysis by using HiC Pro 3D chromatin heat map generation on Hi-Glass and Juice box II) Services in generating the illustrations in Biology by Biorender, Canva and Adobe illustrator. III) Content Writing: Review of literature, Research proposal, Manuscript Proof Reading, Graphs and figures, Presentations. About myself: As an experienced researcher in Molecular Biology, Genomics and Infection Biology, I have developed expertise in the Bioinformatic data analysis of the biological data acquired from the basic and the high throughput techniques. Besides the Bioinformatic skills I have expertise in conventional molecular biology areas which include: Immunology and Infection Biology: 1) Development of recombinant strains in bacterial system by performing gene cloning. 2) Over expression and purification of proteins from the bacterial systems. 3) Immunological characterization of pathogenic bacterial proteins in human macrophage cell lines: a) By recording the pro-inflammatory and anti-inflammatory cytokines. b) Elucidating the inflammatory pathways by detecting the levels of specific markers using Flow cytometry and western blot. Genomics and Epigenetics: 4) Development of strains in eukaryotic system (yeast) by using homologous recombination. 5) Library preparation for NGS (Next generation sequencing) 6) Studying DNA protein interaction by using ChIP seq and ChIP. 7) Elucidating the DNA damage and repair pathways. Throughout my research career I was able to publish my scientific investigations in international peer-reviewed scientific journals. I also achieved nationwide rank 26 in the National Eligibility Test (General Biology) across India. With an experience of 6 years as an active researcher, I have developed an in-depth understanding of fundamentals in : Molecular Biology, Cell Biology, Microbiology, Biochemistry Genetics. Genomics, Chromatin Biology, Immunology, Ecology and Evolution, Biophysics, Virology and Developmental biology. Just drop me a message for more details.